FastQCFastQC Report
Sun 20 Nov 2022
CO_003-COM.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_003-COM.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150793
Sequences flagged as poor quality0
Sequence length25-345
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG43052.8549070580199345No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC42762.8356753960727623No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA40652.695748476388161No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG40522.6871273865497733No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC38912.5803585047051256No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG38312.5405688592971822No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG36872.4450737103181184No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG29131.9317872845556492No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC28911.91719774790607No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT26331.7461022726519135No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG25481.6897336083239938No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT24721.6393333908072654No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG24031.5935752985881309No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC23011.5259329013946272No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG21661.4364061992267545No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT21621.4337535561995585No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC21011.393300750034816No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA20931.3879954639804235No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG20881.3846796601964282No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG19641.3024477263533454No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG19591.29913192256935No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA19241.275921296081383No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG19231.275258135324584No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT17591.1664997712095388No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG17411.154562877587156No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT17211.1412996624511749No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG17111.1346680548831842No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT16921.1220680005040022No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT16491.0935520879616427No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT16441.0902362841776476No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCA15751.0444781919585127No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA15211.0086675110913637No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT14710.9755094732514109No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT14160.9390356316274628No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA14000.9284250595186779No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT13960.9257724164914817No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG13910.9224566127074865No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT13640.9045512722739119No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT13590.9012354684899168No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAG13320.8833301280563421No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCG13090.8680774306499639No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC12910.856140537027581No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG12540.8316035890260158No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG12320.8170140523764367No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT12170.8070666410244507No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT11880.7878349790772782No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT11800.7825296930228857No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT11680.774571763941297No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT11650.7725822816708998No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC11320.750697976696531No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC11220.7440663691285405No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC11190.7420768868581433No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT11050.7327926362629564No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT10800.7162136173429802No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT10550.6996345984230037No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGC10200.6764239719350368No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT10050.6664765605830509No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGG9670.6412764518246868No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA9430.6253605936615095No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT9200.6101078962551312No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC9160.6074552532279349No Hit
GCCGCCTCCCTAATGTTACACACTGACTAG8850.5868972697671643No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG8490.5630234825223982No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG8380.5557287141976086No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG7710.511296943492072No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC7430.4927284423016984No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA7080.4695178158137314No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG6610.4383492602441758No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG6500.4310544919193862No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6350.4211070805674003No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6220.41248599072901265No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5730.37999111364585886No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCC5470.3627489339690834No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG5250.34815939731950424No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4490.297759179802776No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAG4370.28980125072118734No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3520.23343258639326758No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG3230.21420092444609495No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGA2510.16645334995656297No Hit
TCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2310.15319013482058186No Hit
CCGCCTCCCTAATGTTACACACTGACTAG2290.15186381330698373No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGG1950.12931634757581584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)